Centella asiatica contre l'arthrite ?

[audiomaniac]

Attenuation of collagen induced arthritis by Centella asiatica methanol fraction via modulation of cytokines and oxidative stress.
Sharma S1, Gupta R2, Thakur SC1.
Author information
1
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi-110025, India.
2
Lab Oncology, All India Institute of Medical Sciences, New Delhi-110029, India.
Abstract
OBJECTIVE:
To investigate the anti-inflammatory, antioxidant and anti-arthritic effects of Centella asiatica methanolfraction (CaME) on collagen-induced arthritis (CIA), an animal model of rheumatoid arthritis.

METHODS:
Arthritis was induced in female wistar rats by immunization with porcine type II collagen. The CIA rats were treated orally with CaME (50, 150, and 250 mg/kg/day) for 15 d (beginning on day 21 of the experimental period). The clinical, histological, biochemical, and immunological parameters were assessed.

RESULTS:
CaME treatment (150 and 250 mg/kg) significantly attenuated the severity of CIA and reduced the synovial inflammation, cartilage erosion, and bone erosion as evident from both histological and radiographic data. The escalated plasma levels of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 alongwith nitric oxide in CIA rats decreased significantly on CaME treatment. The serum levels of type-II collagen antibody were significantly lower in rats of CaME (150 and 250 mg/kg) treated group than those in the arthritic group. Furthermore, by inhibiting the above mediators, CaME also contributed towards the reversal of the disturbed antioxidant levels and peroxidative damage.

CONCLUSION:
Our results clearly indicate that oral administration of CaME suppresses joint inflammation, cytokine expression as well as antioxidant imbalance, thereby contributing to an amelioration of arthritis severity in CIA rats.

https://www.ncbi.nlm.nih.gov/pubmed/25484009

[audiomaniac]

Titrated extract of Centella asiatica increases hair inductive property through inhibition of STAT signaling pathway in three-dimensional spheroid cultured human dermal papilla cells.
Choi YM1,2, An S2, Lee J2, Lee JH3, Lee JN4, Kim YS5, Ahn KJ6, An IS2, Bae S1.
Author information
1
a Department of Cosmetics Engineering , Research Institute for Molecular-Targeted Drugs, Konkuk University , Seoul , Republic of Korea.
2
b Korea Institute for Skin and Clinical Sciences, Gene Cell Pharm Corporation , Seoul , Republic of Korea.
3
c Laboratory of Molecular Oncology , Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine , Seoul , Republic of Korea.
4
d Department of Cosmetology, Graduate School of Engineering , Konkuk University , Seoul , Republic of Korea.
5
e Department of Image Industry, Graduate School of Engineering , Konkuk University , Seoul , Republic of Korea.
6
f Department of Dermatology , Konkuk University School of Medicine , Seoul , Republic of Korea.
Abstract
Dermal papilla (DP) is a pivotal part of hair follicle, and the smaller size of the DP is related with the hair loss. In this study, we investigated the effect of titrated extract of Centella asiatica (TECA) on hair growth inductive property on 3D spheroid cultured human DP cells (HDP cells). Significantly increased effect of TECA on cell viability was only shown in 3D sphered HPD cells, not in 2D cultured HDP cells. Also, TECA treatment increased the sphere size of HDP cells. The luciferase activity of STAT reporter genes and the expression of STAT-targeted genes, SOCS1 and SOCS3, were significantly decreased. Also, TECA treatment increased the expression of the hair growth-related signature genes in 3D sphered HDP cells. Furthermore, TECA led to downregulation of the level of phosphorylated STAT proteins in 3D sphered HDP cells. Overall, TECA activates the potential of hair inductive capacity in HDP cells.