Effect of Green Coffee Bean Extract on Body Weight of Wistar Strain Rats Fed with High Fat Diet
Siti Nur Husnul Yusmiati Ann Nutr Metab 2019;75(suppl 3): 67
Background/Aims: The prevalence of obesity is increasing worldwide as well as in Indonesia. It is a public health issue and global epidemic. This research aims to determine the effect of
green coffee extract on the weight of Wistar strain rats fed with high-fat diet.
Methods: This research applied a true randomized experimental control design. The Wistar strain rats were grouped into 5 groups, i.e., group 1 (negative control), group 2 (positive
controls fed high-fat diet), group 3 (fed high-fat diet and green coffee extract with dose of 100 mg/kg bw), group 4 (rats fed high-fat diet and green coffee bean extract with dose of 200 mg/Kg bw),
group 5 (rats fed high-fat diet and green coffee extract with dose of 400 mg/kg bw). The obtained data were tested with one-way ANOVA followed by LSD analysis with α = 0.05.
Results: This research proves that green coffee bean extract with a dose of 100, 200 and 400 mg/kg bw can lower weight of rats fed with hight fat diet. Green coffee extract contains active compound of chlorogenic acid that can increase AMPK activity so it has positive impact that can direct the result of metabolite substance, which is useful in decreasing fat synthesis. Chlorogenic acid activates AMPK to inhibit fatty
acid synthesis, and increase fatty acid oxidation.
Conclusion: green coffee bean extract with a dose of 100–400 mg/kg lower weight of rats fed with higt fat diet.