ainsi que les gènes qui protègent des radicaux libres
Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and
dyslipidemic men
Nutrition & Metabolism 2012, 9:45
The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic
subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effects of n-3
PUFAs and their oxidized metabolites are triggered by regulating the expression of genes. Currently, it is uncertain
if the administration of n-3 PUFAs results in different expression changes of genes related to antioxidative
mechanisms in normo- and dyslipidemic subjects, which may partly explain their cardioprotective effects. The aim
of this study was to investigate the effects of n-3 PUFA supplementation on expression changes of genes involved
in oxidative processes.
Methods: Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with fish oil capsules,
providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. Gene expression levels were determined
by whole genome microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR).
Results: Using microarrays, we discovered an increased expression of antioxidative enzymes and a decreased
expression of pro-oxidative and tissue enzymes, such as cytochrome P450 enzymes and matrix metalloproteinases,
in both normo- and dyslipidemic men. An up-regulation of catalase and heme oxigenase 2 in both normo- and
dyslipidemic subjects and an up-regulation of cytochrome P450 enzyme 1A2 only in dyslipidemic subjects could be
observed by qRT-PCR analysis.
Conclusions: Supplementation of normo- and dyslipidemic subjects with n-3 PUFAs changed the expression of
genes related to oxidative processes, which may suggest antioxidative and potential cardioprotective effects of n-3
PUFAs.
